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If you wish to
obtain the USER cloning compatible vectors for use in your own research, please contact the
Fungal Genetic Stock
Center or me by e-mail (rasf(ad)bio.dtu.dk).
Instructions for single step construction of targeted genome modification
vectors by USER friendly cloning and Agrobacterium tumefaciens
mediated transformation can be found on the following pages:
| Standard
vectors for Agrobacterium tumefaciens mediated transformation |
|
Name |
Refence |
Application |
pAg1
|
Constructed by Zhang and co-workers:
"Efficient disruption of a polyketide synthase gene ( pks1) required for
melanin synthesis through Agrobacterium-mediated transformation
of Glarea lozoyensis" Zhang A, Lu P, Dahl-Roshak AM, Paress PS,
Kennedy S, Tkacz JS, An Z. in Mol Genet Genomics. 2003
Feb;268(5):645-55. Epub 2003 Jan 10.
|
General cloning vector |
pAg1-H3
|
Constructed by Zhang and co-workers:
"Efficient disruption of a polyketide synthase gene ( pks1) required for
melanin synthesis through Agrobacterium-mediated transformation
of Glarea lozoyensis" Zhang A, Lu P, Dahl-Roshak AM, Paress PS,
Kennedy S, Tkacz JS, An Z. in Mol Genet Genomics. 2003
Feb;268(5):645-55. Epub 2003 Jan 10.
|
Vector for targeted gene replacement.
Vector construction by Xi-cloning, In-Fusion cloning or classical
restriction enzyme and ligase dependent cloning. |
pAg1-H3E
|
Rasmus J.N. Frandsen, Jens
A. Andersson, Matilde B. Kristensen and Henriette Giese: "Efficient
four fragment cloning for the construction of vectors for targeted gene
replacement in filamentous fungi", BMC Molecular
Biology 2008, 9:70. |
Vector for in locus overexpression.
Vector construction by Xi-cloning, In-Fusion cloning or classical
restriction enzyme and ligase dependent cloning. |
|
USER
vectors for Agrobacterium tumefaciens mediated transformation |
|
Name |
Reference |
Application
Selection marker |
pRF-HU
|
Frandsen et al. BMC Molecular
Biology 2008, 9:70. |
General cloning vector |
Hygromycin resistance |
pRF-HU2
|
Frandsen et al. BMC Molecular
Biology 2008, 9:70. |
Vector for targeted gene replacement. |
Hygromycin resistance |
pRF-HUE
|
Frandsen et al. BMC Molecular
Biology 2008, 9:70. |
Vector for ectopic overexpression of both
endogenic and heterologous genes |
Hygromycin resistance |
pRF-HU2E
|
Frandsen et al. BMC Molecular
Biology 2008, 9:70. |
Vector for in locus overexpression. |
Hygromycin resistance |
pRF-HUEA
|
Manuscript in preparation |
Vector for ectopic overexpression from the
Fg-PKS12 locus (allows colour screening of transformants based on the
lack of aurofusarin) - only for Fusarium species
|
Hygromycin resistance |
|
pTJ-GU2
NEW |
Manuscript in preparation |
Vector for targeted gene replacement.
|
Geneticin (G418) resistance |
|
pTJ-GUEA
NEW |
Manuscript in preparation |
Vector for ectopic overexpression from the
Fg-PKS12 locus (allows colour screening of transformants based on the
lack of aurofusarin) - only for Fusarium species
|
Geneticin (G418) resistance |
|
pRF-BU2
NEW |
Manuscript in preparation |
Vector for targeted gene replacement. |
Basta resistance |
|
