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On this page
you can find information on some of the different Fusarium graminearum
mutants I have made. The information includes pictures,
predicted function, reference to publication and the sequences of the
Agrobacterium tumefaciens vectors used to generate the mutants. All
deletion mutants are
true deletion / replacement mutants, meaning that part of or the whole coding
sequence of the targeted gene has been replaced by the positive selection marker
hygromycin phosphotransferase (hph).
We have chosen to use the abbreviation "aur" to describe the genes in the
aurofusarin gene cluster.
Information on mutants that have not been published is not shown and is marked
with "na".
The pictures
of the cultures is taken from the bottom of the dishes, and are captured after
7 days if not specified otherwise. All cultures have been incubated at 25
°C in
darkness, with the agar plates upside down.
If
you are interested in one or two of the mutants - please contact
raf(at)bio.dtu.dk
|
Name: |
aurR1 |
|
Accession number: |
FG02320.1 |
|
Mutant type: |
Deletion |
|
Domains |
"Binuclear zinc cluster domain" and "Fungal
transcription factor domain" |
|
Vector sequence: |
pAg1-H3::ΔaurR1 |
| |
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
Specific transcription factor for the
aurofusarin gene cluster |
|
Phenotype of mutant: |
The mutant is characterized by a milky white
vegetative mycelium. The lack of aurofusarin biosynthesis is based on a lack
of expression of the gene in the aurofusarin gene cluster.
|
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
Kim et al. 2006 |
|
Back to top |
|
|
Name: |
aurO |
|
Accession number: |
FG02321.1 |
|
Mutant type: |
Deletion |
|
Domains |
FAD linked
oxidase |
|
Vector sequence: |
pAg1-H3::ΔaurO |
|
Vector constructed using Xi-Cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
oxidoreductase
that catalyses the conversion of dimeric 9-hydroxyrubrofusarin to
aurofusarin |
|
Phenotype of mutant: |
Accumulates
rubrofusarin |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurT |
|
Accession number: |
FG02322.1 |
|
Mutant type: |
Deletion |
|
Domains |
Major facilitator
superfamily |
|
Vector sequence: |
pAg1-H3::ΔaurT |
|
Vector constructed using Xi-Cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurR2 |
|
Accession number: |
FG02323.1 |
|
Mutant type: |
Deletion |
|
Domains |
"Binuclear zinc cluster domain" and "Fungal
transcription factor domain" |
|
Vector sequence: |
pAg1-H3::ΔaurR2 |
|
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
Fine tunes the
expression of genes encoding enzymes that converts rubrofusarin to
aurofusarin |
|
Phenotype of mutant: |
Altered ratio
between rubrofusarin and aurofusarin |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
PKS12/aurC
THIS IS NOT A
DESIGNED DOUBLE REPLACEMENT MUTANAT
- IT JUST
HAPPENED:
The
PKS12 genes is rendered non-functional due to a mutation in its coding
sequence, which occured during
integration of the aurC replacement vector. |
|
Accession number: |
FG12040.1 and FG02325.1 |
|
Mutant type: |
Deletion |
|
Domains |
Non |
|
Vector sequence: |
pAg1-H3::ΔaurC
|
|
Vector constructed using Xi-Cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurC |
|
Accession number: |
FG02325.1 |
|
Mutant type: |
Deletion |
|
Domains |
Non |
|
Vector sequence: |
pAg1-H3::ΔaurC |
|
Vector constructed using Xi-Cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurC/aurJ |
|
Accession number: |
FG02325.1/FG02326.1 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pAg1-H3::ΔaurCJ |
|
Vector constructed using restriction enzymes
and ligation and Xi-cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurJ |
|
Accession number: |
FG02326.1 |
|
Mutant type: |
Deletion |
|
Domains |
"O-methyltransferase, family 2" and "Winged helix DNA-binding" and "SAM (and
some other nucleotide) binding motif" |
|
Vector sequence: |
pAg1-H3::ΔaurJ |
|
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
O-methyltransferase that catalyse the methylation of nor-rubrofusarin
resulting in formation of rubrofusarin |
|
Phenotype of mutant: |
Light yellow
vegetative mycelium due to the accumulation of nor-rubrofusarin |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
Kim et al. 2006 |
|
Back to top |
|
|
Name: |
aurF |
|
Accession number: |
FG02327.1 |
|
Mutant type: |
Deletion |
|
Domains |
Flavin-containing
monooxygenase FMO |
|
Vector sequence: |
pAg1-H3::ΔaurF |
|
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
Flavin depend
monooxygenase that catalyses the oxidation of rubrofusarin to
9-hydroxyrubrofusarin |
|
Phenotype of mutant: |
Yellow vegetative
mycelium due to the accumulation of rubrofusarin |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
Kim et al. 2006 |
|
Back to top |
|
|
Name: |
GIP1 |
|
Accession number: |
FG02328.1 |
|
Mutant type: |
Deletion |
|
Domains |
Multicopper
oxidase, type 1 |
|
Vector sequence: |
pAg1-H3::ΔGIP1 |
|
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
|
|
Function: |
Laccase that
catalyse the dimerisation of two 9-hydroxyrubrofusarin in C7 positions |
|
Phenotype of mutant: |
Yellow vegetative
mycelium due to the accumulation of rubrofusarin |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
Kim et al. 2006 |
|
Back to top |
|
|
Name: |
aurS |
|
Accession number: |
FG02329.1 |
|
Mutant type: |
Deletion |
|
Domains |
Beta-Ig-H3/fasciclin |
|
Vector sequence: |
pAg1-H3::ΔaurS |
|
Vector constructed using Xi-Cloning |
|
Picture: |
Mutant on
DFM
Wild type on DFM
Mutant on PDA
Wild type on PDA
|
|
HPLC-DAD data |
na |
|
Function: |
? |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
aurL2 |
|
Accession number: |
FG02330.1 |
|
Mutant type: |
Deletion |
|
Domains |
Multicopper
oxidase, type 1 |
|
Vector sequence: |
pAg1-H3::ΔaurL2 |
|
Vector constructed using restriction enzymes
and ligation |
|
Picture: |
Similar to wild type |
|
HPLC-DAD data |
|
|
Function: |
? (no function in
aurofusarin biosynthesis) |
|
Phenotype of mutant: |
Red vegetative
mycelium similar to the wild type |
|
Reference: |
Frandsen et al. 2006 |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
MET12 |
|
Accession number: |
FG07127 |
|
Mutant type: |
Deletion |
|
Domains |
Methylenetetrahydrofolate reductase |
|
Vector sequence: |
pAg1-H3::Δmet12 |
|
Vector constructed using
Xi-cloning |
|
Picture: |
Wild type (left)
and DFg-MET12
(right) on DFM medium with increasing concentrations of L-methionine
 |
|
HPLC-DAD data |
na |
|
Function: |
Methylenetetrahydrofolate reductase (methionine biosynthesis) |
|
Phenotype of mutant: |
The mutation is
lethal but can be rescued by supplementing the growth medium with exogenous
L-methionine. At low L-methionine concentrations the mutant is unable to
complete aurofusarin biosynthesis and accumulates precursers of aurofusarin.
At higher concentrations (the ability to complete aurofusarin biosynthesis
is restored.
See
publication for details
|
|
Reference: |
"Methylenetetrahydrofolate reductase activity is involved in the plasma
membrane redox system required for pigment biosynthesis in filamentous fungi".
Accepted for publication in Eukaryotic Cell 2010. Rasmus J.N. Frandsen,
Klaus S. Albertsen, Peter Stougaard, Jens L. Sørensen, Kirstian F. Nielsen,
Stefan Olsson, Henriette Giese |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
MET13 |
|
Accession number: |
FG09572
|
|
Mutant type: |
Deletion |
|
Domains |
Methylenetetrahydrofolate reductase |
|
Vector sequence: |
pAg1-H3::Δmet12 |
|
Vector constructed using
Xi-cloning |
|
Picture: |
Wild type (left)
and DFg-MET13
(right) on DFM medium with increasing concentrations of L-methionine
 |
|
HPLC-DAD data |
na |
|
Function: |
Methylenetetrahydrofolate reductase (methionine biosynthesis) |
|
Phenotype of mutant: |
The mutation is
lethal but can be rescued by suplementing the growth medium with exogenous
L-methionine. The strain remains aurofusarin deficinet throughout the tested
L-methionine concentration range.
See
publication for details
|
|
Reference: |
"Methylenetetrahydrofolate reductase activity is involved in the plasma
membrane redox system required for pigment biosynthesis in filamentous fungi".
Accepted for publication in Eukaryotic Cell 2010. Rasmus J.N. Frandsen,
Klaus S. Albertsen, Peter Stougaard, Jens L. Sørensen, Kirstian F. Nielsen,
Stefan Olsson, Henriette Giese |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PGL1 |
|
Accession number: |
na |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
(a) pAg1-HU3::ΔPGL1
and (b) pRF-HU2::ΔPGL1 |
|
Vector constructed by (a)
In-Fusion cloning and (b) USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PGL1 |
|
Accession number: |
na |
|
Mutant type: |
In locus overexpression |
|
Domains |
na |
|
Vector sequence: |
(a) pAg1-H3E::PGL1 and
(b) pRF-HU2E::OPGL1 |
|
Vector constructed by (a)
In-Fusion cloning and (b) USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
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|
Back to top |
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|
Name: |
pglR |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUE:pglR |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
pglJ |
|
Accession number: |
na |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::ΔpglJ |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
pglM |
|
Accession number: |
na |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::ΔpglM |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
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|
Back to top |
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|
Name: |
pglX |
|
Accession number: |
na |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::ΔpglX |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
pglV |
|
Accession number: |
na |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::ΔpglV |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
pglR (PKS3-R3) |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::pglR |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
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|
Back to top |
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|
Name: |
PKS5-R1 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS5-R1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
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|
Name: |
PKS5-R2 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS5-R2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS7-R1 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS7-R1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS7-R2 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS7-R2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS9-R1 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS9-R1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS9-R2 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS9-R2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS11-R1 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS11-R1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS15-R1 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS15-R1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS15-R2 |
|
Accession number: |
na |
|
Mutant type: |
Ectopic
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HUEA::PKS15-R2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
|
|
Back to top |
|
|
Name: |
PKS1 |
|
Accession number: |
fg10548 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS1 |
|
Accession number: |
fg10548 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS1 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS2 |
|
Accession number: |
fg12977 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005
|
|
Back to top |
|
|
Name: |
PKS2 |
|
Accession number: |
fg12977 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS2 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS5 |
|
Accession number: |
fg05794 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS5 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS5 |
|
Accession number: |
fg05794 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS5 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS6 |
|
Accession number: |
fg12109 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS6 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS6 |
|
Accession number: |
fg12109 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS6 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS7 |
|
Accession number: |
fg08795 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS7 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS7 |
|
Accession number: |
fg08795 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS7 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS8 |
|
Accession number: |
fg03340 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS8 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS8 |
|
Accession number: |
fg03340 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS8 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS9 |
|
Accession number: |
fg12121 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS9 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS9 |
|
Accession number: |
fg12121 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS9 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS10 |
|
Accession number: |
fg12100 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::vPKS10 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS10 |
|
Accession number: |
fg12100 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS10 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS11 |
|
Accession number: |
fg01790 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS11 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS11 |
|
Accession number: |
fg01790 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS11 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS14 |
|
Accession number: |
fg03964 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS14 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS14 |
|
Accession number: |
fg03964 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS14 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS15 |
|
Accession number: |
fg04588 |
|
Mutant type: |
Deletion |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2::∆PKS15 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
Name: |
PKS15 |
|
Accession number: |
fg04588 |
|
Mutant type: |
In locus
overexpression |
|
Domains |
na |
|
Vector sequence: |
pRF-HU2E::PKS15 |
|
Vector constructed using USER cloning |
|
Picture: |
na |
|
HPLC-DAD data |
na |
|
Function: |
na |
|
Phenotype of mutant: |
na |
|
Reference: |
Not published |
|
Similar mutants: |
Gaffoor et al. 2005 |
|
Back to top |
|
|
