Molecular biology


Experimental setup
ATMT of new fungi
Molecular biology
Growth media



Cloning techniques

  USER Friendly cloning

      Preparing USER vectors for cloning

      Cloning of two PCR amplicons into two USER cloning sites (UCS)

  Xi-cloning The cheapest system for directional blunt-end cloning (no kits nor ligase - just primers)

  In-Fusion cloning Very efficient system for directional cloning of single inserts (but less efficient for multi fragment cloning)



  Agrobacterium tumefaciens mediated transformation of filamentous fungi

  Production and use of chemical competent E. coli cells

  Production and use of electrocompetent E. coli cells

  Production and use of electrocompetent A. tumefaciens cells


Crossing of Fusarium graminearum strains

Plug analysis of secondary metabolite production (extraction)

Fast extraction of genomic DNA from filamentous fungi

Purifying macroconidia from Fusarium




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Dette sted blev sidst opdateret 12. July 2010