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Cloning techniques
USER Friendly cloning
Preparing USER vectors for cloning
Cloning of two PCR amplicons into two USER cloning sites (UCS)
Xi-cloning
The
cheapest system for directional blunt-end
cloning (no kits nor ligase - just
primers)
In-Fusion cloning
Very
efficient system for directional cloning of single inserts (but
less efficient for multi fragment cloning)
Transformation
Agrobacterium tumefaciens
mediated transformation of filamentous fungi
Production and use of
chemical competent E. coli cells
Production and use of
electrocompetent E. coli cells
Production and use of
electrocompetent A. tumefaciens cells
Crossing of Fusarium
graminearum strains
Plug analysis of secondary
metabolite production (extraction)
Fast extraction of genomic
DNA from filamentous fungi
Purifying macroconidia from
Fusarium
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