Curriculum vitae

01/08/10

Name:     Rasmus John Normand Frandsen
    Born:       February 18th 1979 (Copenhagen, Denmark)
     Spouse:   Mette Frandsen (Laboratory technician in the biotech company Bioneer:FARMA)
   Daughter:   Natasja Normand Frandsen (June 19th 2009)
    Parents: Marianne Frandsen and Flemming Frandsen

Work address:

DTU Systems Biology
Department of Systems Biology
Center for Microbial Biotechnology
Technical University of Denmark
Søltofts plads
Building 223, room 225
2800 Kgs. Lyngby
Denmark

rasf(at)bio.dtu.dk

Employments

	Post Doc fellow at the Center for Microbial Biotechnology (CMB), Department of Systems Biology, Technical University of Denmark
	Ph.D. student at the Department of Ecology, Faculty of Life Sciences, University of Copenhagen, Denmark (2007 – 2010).
	Scientific assistant to Professor Henriette Giese at the Department of Ecology, The Royal Veterinary and Agricultural University, Denmark (2006 – 2007)
	Scientific assistant to Associated Professor Mette Nicolaisen at the Department of Ecology, The Royal Veterinary and Agricultural University, Denmark (2005)
	Scientific assistant to Associated Professor Kristian Brandt Koefoed at the Department of Ecology, The Royal Veterinary and Agricultural University, Denmark (2005)

Education

	Ph.D. in Molecular fungal genetics from the “Faculty of Life Sciences”, “University of Copenhagen”, Denmark, with Professor Henriette Giese as primary supervisor (2006 – 2010).
	Cand. scient. in Biology - Biotechnology from The Royal Veterinary and Agricultural University, Denmark (2002 - 2005). Thesis on the biosynthesis of aurofusarin and the transcriptional regulation of the corresponding gene cluster in Fusarium graminearum
	Bachelor degree in Biology from the University of Aarhus, Denmark (1999 - 2002).

Awards and Scholarships

Young Elite Researcher Price 2010

Novozymes Scholarship 2004 – 2005

Publications

"Methylenetetrahydrofolate reductase activity is involved in the plasma membrane redox system required for pigment biosynthesis in filamentous fungi". Accepted for publication in Eukaryotic Cell 2010. Rasmus J.N. Frandsen, Klaus S. Albertsen, Peter Stougaard, Jens L. Sørensen, Kirstian F. Nielsen, Stefan Olsson, Henriette Giese. DOI:10.1128/EC.00031-10

"A pre-processing strategy for liquid chromatography time-of-flight mass spectrometry metabolic fingerprinting data". Accepted for publication in Metabolomics 2010. Nikoline J. Nielsen, Giorgio Tomasi, Rasmus J. N. Frandsen, Matilde B. Kristensen, John Nielsen, Henriette Giese and Jan H. Christensen DOI: 10.1007/s11306-010-0211-1

"Development of pollution-induced community tolerance is linked to structural and functional resilience of a soil bacterial community following a five-year field exposure to copper". Soil Biology & Biochemistry 2010, 42:748-757.Brandt K.K., Frandsen R.J.N., Holm P.E. and Nybroe O.

"Applying Parallel Factor Analysis models to HPLC diode array detector datasets reveals strain dependent regulation of polyketide biosynthesis in Fusarium graminearum, Fusarium culmorum and Fusarium pseudograminearum". Anal Chim Acta. 2009, 647(2):243-8. Andersson J.A., Andersson C.A. and Frandsen R.J..

"Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi". BMC Molecular Biology 2008, 9:70. Rasmus J.N. Frandsen*, Matilde Bylov Kristensen, Jens A. Andersson and Henriette Giese (link to PDF)

"The biosynthetic pathway for aurofusarin in Fusarium graminearum reveals a close link between the naphthoquinones and naphthopyrones". Molecular Microbiology, 2007, 61(4):1069-1080. Rasmus J. N. Frandsen*, Nikoline J. Nielsen, Nicolai Maolanon, Jens C. Sørensen, Stefan Olsson, John Nielsen and Henriette Giese. (pdf's: article and supplimentry material)

"The PKS4 gene of Fusarium graminearum is essential for zearalenone production". Applied and Environmental Microbiology, 2006, 72(6):3924-3932. Lysoe E., Klemsdal S.S., Bone K.R., Frandsen R.J.N., Johansen T., Thrane U. and Giese H. (pdf: article)

Invited reviewer for

	Journal of Agricultural and Food Chemistry
	European Journal of Plant Pathology
	BioTechniques
	Bioresource Technology

Invited speaker at

	"Fusarium Workshop" at the 25th Fungal Genetics Conference at Asilomar USA, 2009, 15 min oral presentation "Gene Replacement Technology in Fusarium graminearum".
	“10th International Fusarium Workshop and the International Fusarium Genomics Workshop” in Alghero, Italy 2008. 15 min. oral presentation "Identification of the gene cluster and pathway specific transcription factor responsible for perithecium pigment biosynthesis is Gibberella zeae"
	“NORDFORSK Network on New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe”, meeting at Ås, Norway, 2007. 20 minutes talk: “The aurofusarin biosynthesis is dependent on a methylenetetrahydrofolate reductase, encoded by a gene located outside the aurofusarin gene cluster”
	“The 8th Conference of the European Foundation for Plant Pathology & the British Society for Plant Pathology Presidential Meeting”, Copenhagen, Denmark 2006. 20 min. oral presentation: ”The aurofusarin biosynthesis in Fusarium graminearum”.
	“International Fungal Genetics Conference”, Copenhagen, Denmark, 2005. 30 min. oral presentation: ”Theory on the biosynthesis of aurofusarin”.

Posters

	"Construction of vectors for targeted gene replacement in fungi by USER Friendly cloning" at the 25th Fungal Genetics Conference at Asilomar USA, 2009, by Rasmus J.N. Frandsen, Jens A. Andersson, Matilde B. Kristensen and Henriette Giese
	*"New vector for expression of homologous and heterologous genes in Fusarium graminearum, allowing for colour based screening of transformants*" at the yearly Plant Biotech Denmark conference held at University of Copenhagen, Faculty of Life Sciences, January 2009, by Rasmus J.N. Frandsen and Henriette Giese
	"Highly efficient single step construction of vectors for targeted genome modifications in filamentous fungi by USER Friendly cloning" at " The 10th International Fusarium Workshop and the International Fusarium Genomics Workshop” in Alghero, Italy 2008.
	“USER Friendly construction of vectors for targeted gene replacement in fungi” at "The 9th European Conference on Fungal Genetics” (ECFG9), Edinburgh, Scotland, 2008.
	”Impact of previous long-term copper field-exposure on resistance of soil microbial communities to experimentally increased copper levels” at the International Symposium on Microbial Ecology (ISME12), Cairns, Australia, 2008, by Brandt KK, Frandsen RJN, Holm PE, Nybroe O
	“USER Friendly construction of vectors for targeted gene replacement in fungi” at "Annual Plant Biotech Denmark Meeting” (PBD), Copenhagen, Denmark 2008.
	”Role of Fusarium expansin-like proteins in cereal infection” at the VAAM Symposium "Molecular Biology of Fungi" in Hamburg 2007. Rasmussen JJ, Frandsen RJN, Giese H.
	"Applying PARAFAC models to detect new regulatory mechanisms in Fusaria" at “The 24th Fungal Genetics Conference at Asilomar”, California, USA, 2007, by Andersson JA, Andersson CA, Frandsen RJN.
	“AurS and AurC: Two conserved hypothetical proteins required for aurofusarin biosynthesis in Fusarium graminearum” at the “Congress of European Mycologists”, St. Petersburg, Russia, 2007, by Frandsen RJN, Nielsen NJ, Nielsen J, Giese H.
	"Analysis of Methylene TetraHydrofolate Reductases (MTHR) in filamentous fungi" at Plant Biotech Denmark Annual Meeting 2007, by Frandsen RJN, Giese H.
	“Analysis of methylenetetrahydrofolate reductases (MTHR) in filamentous fungi” at “The 24th Fungal Genetics Conference at Asilomar”, California, USA, 2007, by Frandsen RJN and Giese H.
	“The transcriptional regulation of the aurofusarin gene cluster” at “The 8th European Conference on Fungal Genetics” (ECFG8), Vienna, Austria, 2006. By Albertsen KS, Andersson JA, Frandsen RJN, Giese H.

Congresses I have attended

	"the 25th Fungal Genetics Conference at Asilomar", California, USA, 2009
	“Annual Plant Biotech Denmark Meeting” (PBD), Copenhagen, Denmark, 2009
	“10th International Fusarium Workshop and the International Fusarium Genomics Workshop” in Alghero, Italy 2008.
	“The 9th European Conference on Fungal Genetics” (ECFG9), Edinburgh, Scotland, 2008.
	“Annual Plant Biotech Denmark Meeting” (PBD), Copenhagen, Denmark 2008.
	“Congress of European Mycologists”, St. Petersburg, Russia, 2007.
	“The 24th Fungal Genetics Conference at Asilomar”, California, USA, 2007.
	“NORDFORSK Network on New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe”, meeting at Ås, Norway, 2007.
	The “*9th European Fusarium* Seminar**” in Wageningen, The Netherlands, 2006.
	“The 8th Conference of the European Foundation for Plant Pathology & the British Society for Plant Pathology Presidential Meeting”, Copenhagen, Denmark 2006.
	“The 8th European Conference on Fungal Genetics” (ECFG8), Vienna, Austria, 2006.
	“*The 4th International Fusarium* Genomics Workshop**” in Vienna, Austria, 2006.
	“International Fungal Genetics Conference”, Copenhagen, Denmark, 2006.
	“The 7th European Conference on Fungal Genetics” (ECFG7) at the Danish Technical University, Lyngby, Denmark, 2004.
	“*The 3rd International Fusarium* Genomics Workshop**”, Copenhagen, Denmark, 2004.

Students I have assisted in supervising:

Name (periode)	Subject	Type of student
Andreas H. Klem (2009)	PKS gene cluster	Bachelor student (15 ECTS)
Maria Benn Hansen (2008)	NRPS	Bachelor student (15 ECTS)
Marie Neergaard Harder (2008)	PKS's	Bachelor student (15 ECTS)
Jan Kjølhede Vester (2008)	PKS gene cluster	Bachelor student (15 ECTS)
Janus Jagd Rasmussen (2007 – 2008)	Heterologous expression	Master student (60 ECTS)
Susanne Svensen (2007)	General laboratory skills	Laboratory technician trainee
Anne Stilling (2007)	Novel pathogenicity factor	Bachelor student (15 ECTS)
Mette Sidsel Mortensen (2007	Novel pathogenicity factor	Bachelor student (15 ECTS)
Barbara Chomentowska (2007)	Transcription factors	Theme student (24 ECTS)
Katarzyna Mosiniak (2007)	Transcription factors	Theme student (24 ECTS)
Matilde Bylov Kristensen (2006 – 2007)	NRPS	Master student (60 ECTS)
Nicolai Nareth Maolanon (2006)	Aurofusarin	Bachelor student (15 ECTS)
Terese Jensen (2006)	Sexual crossing	Theme student (24 ECTS)
Christa Kaltoft (2006)	Sexual crossing	Theme student (24 ECTS)
Maria H. Engelhardt (2006)	Sexual crossing	Theme student (24 ECTS)
Jeanette Jensen (2006)	Sexual crossing	Theme student (24 ECTS)
Pia Petersen (2005)	General laboratory skills	Laboratory technician trainee
Kristine Slot Ravnholt (2005 – 2006)	NRPS	Master student (60 ECTS)
Susanne Iversen (2005)	General laboratory skills	Laboratory technician trainee
Tine Manon Jensen (2004)	Aurofusarin	Theme student (24 ECTS)
Steffen E Sørensen(2004)	Aurofusarin	Theme student (24 ECTS)
Jeannette Jensen (2004)	Aurofusarin	Theme student (24 ECTS)

Teaching

	NORDFORSK Ph.D. course: "Techniques for perfoming targeted genome modifications in fungi", Organized by Professor Dan Funck Jensen December 2008
	NORDFORSK Ph.D. course: “Fusarium, genes and toxins”, Organized by Sonja Klemsdal, June 21 – 28, 2008, Ås, Norway
	Course: One lecture at the “Genetic 2” summer 2007 (app. 50 students) held at the University of Copenhagen - Faculty for Life Sciences, Denmark
	Course: Responsible for one experiment “Genetic 2” summer 2006 (app. 50 students) held at The Royal Veterinay and Agricultural University, Denmark
	Course: Two lectures at the “Biotechnology theme” 2007 (app. 10 students) held at The Royal Veterinay and Agricultural University, Denmark
	Course: Two lectures at the “Biotechnology theme” 2006 (app. 15 students) held at The Royal Veterinay and Agricultural University, Denmark
	Course: “Analysis and 3D-modelling of proteins” as Tutor/teachers aid to Professor Morten J. Bjerrum held at The Royal Veterinay and Agricultural University, Denmark, 2004

Memberships

	NORDFORSK Network on New Emerging Mycotoxins and Secondary Metabolites in Toxigenic Fungi of Northern Europe.
	Member of "BioteknoLogen" - a fraternity for Ph.D's, Ph.D. studens and M.Sc primary graduated from Royal Veterinary and Agricultural University, Denmark (2005- ).
	Member of "Ungdommens Naturvidenskabelige Forening" (2000-2002 and 2006-2007).
	Part of the LinkedIn network.

Dette sted blev sidst opdateret 01. August 2010